Plasmid_Backbone

Part:BBa_J107055:Design

Designed by: Lorenzo Pasotti   Group: Magni Lab   (2011-08-12)

pSB3K3 with RBS-RFP-TT between SpeI-PstI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3598
    Illegal NheI site found at 2253
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal NotI site found at 878
    Illegal NotI site found at 3604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3598
    Illegal XhoI site found at 1046
    Illegal XhoI site found at 1889
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3598
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3598
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3613
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal AgeI site found at 581
    Illegal AgeI site found at 693
    Illegal AgeI site found at 2339
    Illegal AgeI site found at 2662
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 3367


Design Notes

This is not a standard BioBrick plasmid, as the suffix is not SpeI-NotI-PstI.


Source

pSB3K3 (backbone) and BBa_J61002 (SpeI-PstI insert).

References